Zearalenone Colloidal Gold Rapid Detection Card

Zearalenone Colloidal Gold Rapid Detection Card

operation instructions

(Colloidal gold method)

1. Principle and Application

This product is made by the principle of competitive inhibition colloidal gold immunochromatography, and is used to detect Zearalenone (ZEN) in grains, feedstuffs and other samples.

After the sample solution is dropped into the sampling hole of the detection card, the Zearalenone in the sample solution is combined with the gold labeled antibody, thus preventing the gold labeled antibody from combining with the Zearalenone conjugate on the cellulose membrane. When the content of Zearalenone in the sample solution is greater than the detection limit, the detection line does not show color, and the result is positive; When the content of Zearalenone in the sample solution is less than the detection limit, the detection line shows purple red, and the result is negative.

2. Technical indicators

2.1 Sensitivity of reagent card: 10ppb (ng/ml)

The final detection limit for the sample must be determined by multiplying the sensitivity of the reagent card by the dilution ratio of the sample treatment.

2.2 Lower limit of sample detection:

Cereals, feed (not blown dry)...... 100ppb

Cereals, feed (blow dried)...... 60ppb

Grain and oil

3. Kit composition

Detection card: 40 pieces/box

Instructions: 1 copy

4. Required equipment and reagents

4.1 Instruments: homogenizer, oscillator, centrifuge, graduated pipette, balance (sensitivity 0.01g)

4.2 Micro Pipette: single channel 20 µ l-200 µ l, 100 µ l-1000 µ l

4.3 Reagent: Methanol

5. Sample pretreatment

5.1 Instructions before sample processing:

The experimental equipment must be clean and use a disposable suction head to avoid contamination and interference with the experimental results.

5.2 Sample preprocessing steps:

5.2.1 Cereals and feed

1) Weigh 2g of crushed sample into a 50ml centrifuge tube, and add methanol according to different detection limit requirements in the following table

Shake for 5 minutes at room temperature of 4000 rpm/separation heart for 5 minutes.

2) Take 0.15ml of supernatant, add 0.85ml of deionized water, mix well and set aside.

5.2.2 Cereals and feed (some highly absorbent samples, detection limit: 100ppb)

1) Weigh 2g of the crushed sample into a 50ml centrifuge tube, add 5ml of 75% methanol, shake for 5 minutes, and separate at room temperature of 4000 rpm for 5 minutes.

2) Take 0.15ml of supernatant, add 0.45ml of deionized water, mix well and set aside.

5.2.3 Grain and feed (detection limit: 60ppb, need to be blown dry)

1) Weigh 2g of crushed sample into a 50ml centrifuge tube, add 4ml of methanol, shake for 5 minutes, and separate at room temperature of 4000 rpm for 5 minutes.

2) Take 1ml of the upper liquid and dry it with nitrogen or air at 50-60 ℃;

3) Dissolve the remaining residue in 0.45ml of methanol and shake vigorously for 2 minutes;

4) Take 0.15ml of the shaken liquid, add 0.85ml of deionized water, and mix well for later use.

5.2.4 Grain and oil (detection limit: 100ppb)

1) Weigh 2g of the sample into a 50ml centrifuge tube, add 4ml of n-hexane and 3ml of methanol, shake for 5 minutes, and separate at room temperature of 4000 rpm for 5 minutes.

2) Take 0.15ml of the upper liquid, add 0.85ml of deionized water, and mix well for later use.

6. Experimental Steps

6.1 Tear open the aluminum foil packaging bag of the inspection card, take out the inspection card, and place it on a flat and clean tabletop.

6.2 Suck the prepared sample liquid with a matching pipette, and slowly drop 2-3 drops (about 60ul) into the sample hole (S) one by one (foam should be avoided).

6.3 Leave at room temperature for 8-10 minutes to determine the results. Results exceeding 10 minutes can only be used as a reference.

7. Result judgment

Negative: Both the C and T lines show color, indicating that the concentration in the sample is below the detection limit or does not contain.

Positive: The C line shows red, while the T line does not show color, indicating that the concentration in the sample is above the detection limit.

Invalid: The absence of a quality control C-line indicates an incorrect operating process or a failure of the detection card.

8. Precautions

8.1 Products that have expired or have damaged aluminum foil bags cannot be used.

8.2 When removing the detection card from the refrigerator, it should be restored to room temperature before opening. The opened detection card should be used as soon as possible to avoid failure due to moisture.

8.3 Do not touch the white film surface in the center of the detection card.

8.4 Droppers should not be mixed to avoid cross contamination.

8.5 The sample solution to be tested should be clear, free from turbid particles, and bacterial contamination, otherwise it may lead to abnormal phenomena such as blockage and unclear color development, which may affect the judgment of experimental results.

9. Storage and shelf life

Storage conditions: The reagent kit should be stored in a dry environment at 2-30 ℃.

Shelf life: The product has a validity period of 1 year, and the production date can be found in the packaging box.